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anti wisp1 antibodies  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti wisp1 antibodies
    Anti Wisp1 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+wisp1+antibody/WISP-1+Antibody/pm39468941-57-31-33
    Average 93 stars, based on 33 article reviews
    anti wisp1 antibodies - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: WISP1/CCN4 inhibits adipocyte differentiation through repression of PPARγ activity
    Article Snippet: Adipocyte culture media collected during adipocyte differentiation and cell extracts were supplemented with protease inhibitor cocktail (Merck Millipore), and equal amounts of protein (100 μg/lane) were loaded onto SDS-PAGE gels. .. After transfer onto nitrocellulose membrane, blots were incubated overnight at 4 °C with anti-WISP1 antibody (1/250, Santa Cruz Biotechnology), anti-β-catenin antibody (1/1000, Santa Cruz Biotechnology), anti-actin-HRP antibody (1/2000, Santa Cruz Biotechnology), anti-adiponectin antibody (1/1000, Pierce), anti-active-β-catenin antibody (1/1000, phospho-β-Catenin (Ser675), Cell Signaling) or anti-Flag-M2 (1/1000, Sigma) and followed by incubation with a horseradish peroxidase-conjugated secondary antibody (1/2000, Cell Signaling). .. Bands were revealed with an enhanced chemiluminescence detection system (Millipore) and visualized on Chemidoc systems (Biorad) .

    Incubation:

    Article Title: WISP1/CCN4 inhibits adipocyte differentiation through repression of PPARγ activity
    Article Snippet: Adipocyte culture media collected during adipocyte differentiation and cell extracts were supplemented with protease inhibitor cocktail (Merck Millipore), and equal amounts of protein (100 μg/lane) were loaded onto SDS-PAGE gels. .. After transfer onto nitrocellulose membrane, blots were incubated overnight at 4 °C with anti-WISP1 antibody (1/250, Santa Cruz Biotechnology), anti-β-catenin antibody (1/1000, Santa Cruz Biotechnology), anti-actin-HRP antibody (1/2000, Santa Cruz Biotechnology), anti-adiponectin antibody (1/1000, Pierce), anti-active-β-catenin antibody (1/1000, phospho-β-Catenin (Ser675), Cell Signaling) or anti-Flag-M2 (1/1000, Sigma) and followed by incubation with a horseradish peroxidase-conjugated secondary antibody (1/2000, Cell Signaling). .. Bands were revealed with an enhanced chemiluminescence detection system (Millipore) and visualized on Chemidoc systems (Biorad) .

    SDS Page:

    Article Title: WNT1-Inducible Signaling Pathway Protein 1 Contributes to Ventilator-Induced Lung Injury
    Article Snippet: .. Samples were heated (100°C; 5 min) and separated by 10% SDS-PAGE followed by immunoblot with anti-TLR4 antibody (sc-10741, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or anti-WISP1 antibody (MAB1680, R&D Systems, Minneapolis, MN, USA), respectively. .. Peritoneal macrophages were obtained with PBS containing 5 mM EDTA (Sigma-Aldrich, St Louis, MO, USA) by repeated peritoneal lavage.

    Western Blot:

    Article Title: WNT1-Inducible Signaling Pathway Protein 1 Contributes to Ventilator-Induced Lung Injury
    Article Snippet: .. Samples were heated (100°C; 5 min) and separated by 10% SDS-PAGE followed by immunoblot with anti-TLR4 antibody (sc-10741, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or anti-WISP1 antibody (MAB1680, R&D Systems, Minneapolis, MN, USA), respectively. .. Peritoneal macrophages were obtained with PBS containing 5 mM EDTA (Sigma-Aldrich, St Louis, MO, USA) by repeated peritoneal lavage.



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    Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
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    Differential effects of pre- and post-loading urine samples on the expression of <t>WISP1,</t> MMP9, Runx2, and TGFβ in EO771 cells. Of note, pre-S & post-S = pre- & post-step aerobics, and pre-L & post-L = pre- and post-loading. The bar chart is based on 10 pairs of human samples and 10 pairs of mouse samples. (A&B) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to human post-step aerobics urine samples. (C&D) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to murine post-knee loading urine samples.
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    Image Search Results


    Figure 2. Reverse translational analysis links WISP1 and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.

    Journal: Cell metabolism

    Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

    doi: 10.1016/j.cmet.2022.07.009

    Figure Lengend Snippet: Figure 2. Reverse translational analysis links WISP1 and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

    Techniques: Expressing, Western Blot, Control, Gene Expression, Transfection, Live Cell Imaging, Migration

    Figure 3. Wisp1 is required for liver fibrosis progression, but not initiation, in CCl4 model (A) Top 20 GO biological processes enriched in the upregulated genes in livers from 6-week CCl4 model. (B) Heatmap of Wisp1 and cytoskeleton gene expression in livers from 6-week CCl4 model, n = 6 mice/group. (C) Experimental regime of CCl4 model in WT and Wisp1 KO mice at indicated timepoints: 3 weeks (oil/WT, n = 3; CCl4/WT, n = 9; oil/KO, n = 5; CCl4/KO, n = 10) and 6 weeks (oil/WT, n = 4; CCl4/WT, n = 10; oil/KO, n = 4; CCl4/KO, n = 11). (D) Representative trichrome staining images of livers after 3- or 6-week CCl4 treatment; scale bars, 500 mm. (E and F) Quantification of fibrosis by relative lesion size (E) and hydroxyproline (F). (G) Representative western blot of type I collagen and cytoskeleton proteins in liver lysates after 6-week CCl4 treatment. GAPDH is a loading control.

    Journal: Cell metabolism

    Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

    doi: 10.1016/j.cmet.2022.07.009

    Figure Lengend Snippet: Figure 3. Wisp1 is required for liver fibrosis progression, but not initiation, in CCl4 model (A) Top 20 GO biological processes enriched in the upregulated genes in livers from 6-week CCl4 model. (B) Heatmap of Wisp1 and cytoskeleton gene expression in livers from 6-week CCl4 model, n = 6 mice/group. (C) Experimental regime of CCl4 model in WT and Wisp1 KO mice at indicated timepoints: 3 weeks (oil/WT, n = 3; CCl4/WT, n = 9; oil/KO, n = 5; CCl4/KO, n = 10) and 6 weeks (oil/WT, n = 4; CCl4/WT, n = 10; oil/KO, n = 4; CCl4/KO, n = 11). (D) Representative trichrome staining images of livers after 3- or 6-week CCl4 treatment; scale bars, 500 mm. (E and F) Quantification of fibrosis by relative lesion size (E) and hydroxyproline (F). (G) Representative western blot of type I collagen and cytoskeleton proteins in liver lysates after 6-week CCl4 treatment. GAPDH is a loading control.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

    Techniques: Gene Expression, Staining, Western Blot, Control

    Figure 4. WISP1 induces cytoskeleton and motility reprogramming via multiple integrins (A) Representative western blots of hWISP1 induced MRTFA and MRTFB nuclear translocation in HSCs in the presence ofArg-Gly-Asp-Ser (RGDS) or Arg-Gly- Glu-Ser (RGES) peptides. YY1 is a loading control for nuclear fractions, and GAPDH is a loading control for whole-cell lysates. (B) hWISP1 induced cytoskeleton gene expression in the presence of RGDS or RGES peptides in HSCs, n = 3 biological replicates. (C) hWISP1 induced HSC migration in the presence of RGDS or RGES peptides, n = 4 biological replicates. (D–F) hWISP1 induced migration (D), MRTFA and MRTFB nuclear translocation (E), and cytoskeleton gene expression (F) of HSCs transfected with siRNAs tar- geting integrins as indicated, n = 4 (D) or 3 (F) biological replicates. (G and H) RHOA activation (G) and F-actin formation (H) in HSCs stimulated with hWISP1 in the presence of RGDS or RGES peptides, n = 3 (G) or 4 (H) biological replicates. (I and J) RHOA activation (I) and F-actin formation (J) in HSCs transfected with siRNAs targeting integrins as indicated, n = 3 (I) or 4 (J) biological replicates. Data in (B)–(J) except (E) represent mean ± SEM, and similar results were seen in at least three independent experiments. p value is calculated using: (C, F, I, and J) two-way ANOVA and (B, D, G, and H) one-way ANOVA. See also Figure S9.

    Journal: Cell metabolism

    Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

    doi: 10.1016/j.cmet.2022.07.009

    Figure Lengend Snippet: Figure 4. WISP1 induces cytoskeleton and motility reprogramming via multiple integrins (A) Representative western blots of hWISP1 induced MRTFA and MRTFB nuclear translocation in HSCs in the presence ofArg-Gly-Asp-Ser (RGDS) or Arg-Gly- Glu-Ser (RGES) peptides. YY1 is a loading control for nuclear fractions, and GAPDH is a loading control for whole-cell lysates. (B) hWISP1 induced cytoskeleton gene expression in the presence of RGDS or RGES peptides in HSCs, n = 3 biological replicates. (C) hWISP1 induced HSC migration in the presence of RGDS or RGES peptides, n = 4 biological replicates. (D–F) hWISP1 induced migration (D), MRTFA and MRTFB nuclear translocation (E), and cytoskeleton gene expression (F) of HSCs transfected with siRNAs tar- geting integrins as indicated, n = 4 (D) or 3 (F) biological replicates. (G and H) RHOA activation (G) and F-actin formation (H) in HSCs stimulated with hWISP1 in the presence of RGDS or RGES peptides, n = 3 (G) or 4 (H) biological replicates. (I and J) RHOA activation (I) and F-actin formation (J) in HSCs transfected with siRNAs targeting integrins as indicated, n = 3 (I) or 4 (J) biological replicates. Data in (B)–(J) except (E) represent mean ± SEM, and similar results were seen in at least three independent experiments. p value is calculated using: (C, F, I, and J) two-way ANOVA and (B, D, G, and H) one-way ANOVA. See also Figure S9.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

    Techniques: Western Blot, Translocation Assay, Control, Gene Expression, Migration, Transfection, Activation Assay

    Figure 5. Discovery of a WISP1-blocking antibody with therapeutic benefits (A and B) Biacore sensorgram plots of anti-WISP1 binding to hWISP1 (A) or mWISP1 (B). (C) Dose-dependent inhibitory activity of anti-WISP1 against hWISP1 induced HSC migration, n = 4 biological replicates.

    Journal: Cell metabolism

    Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

    doi: 10.1016/j.cmet.2022.07.009

    Figure Lengend Snippet: Figure 5. Discovery of a WISP1-blocking antibody with therapeutic benefits (A and B) Biacore sensorgram plots of anti-WISP1 binding to hWISP1 (A) or mWISP1 (B). (C) Dose-dependent inhibitory activity of anti-WISP1 against hWISP1 induced HSC migration, n = 4 biological replicates.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

    Techniques: Blocking Assay, Binding Assay, Activity Assay, Migration

    Figure 6. Therapeutic benefits of WISP1 blocking antibody in CDA-HFD NASH model (A) Unsupervised hierarchical clustering analysis of 25-gene NASH fibrosis signature gene expression in livers from mice fed with normal chow and CDA-HFD diet (8 weeks and 16 weeks). (B) Dosing regime in the preventive (weeks 0–8) and therapeutic (weeks 9–16) CDA-HFD NASH model: n = 10 mice for each group. (C) Representative Sirius red staining images of livers; scale bars, 250 mm. (D and E) Quantification of fibrosis by relative lesion size (D) and newly synthesized hydroxyproline (E). (F) Representative western blots of type I collagen and cytoskeleton proteins in liver lysates from the therapeutic CDA-HFD NASH model. GAPDH is a loading control. (G and H) Representative images (G) and quantification (H) of ACTA2 immunofluorescence in livers; scale bars, 100 mm. Data in (D), (E), and (H) represent mean ± SEM. p value is calculated using one-way ANOVA. See also Figure S11.

    Journal: Cell metabolism

    Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

    doi: 10.1016/j.cmet.2022.07.009

    Figure Lengend Snippet: Figure 6. Therapeutic benefits of WISP1 blocking antibody in CDA-HFD NASH model (A) Unsupervised hierarchical clustering analysis of 25-gene NASH fibrosis signature gene expression in livers from mice fed with normal chow and CDA-HFD diet (8 weeks and 16 weeks). (B) Dosing regime in the preventive (weeks 0–8) and therapeutic (weeks 9–16) CDA-HFD NASH model: n = 10 mice for each group. (C) Representative Sirius red staining images of livers; scale bars, 250 mm. (D and E) Quantification of fibrosis by relative lesion size (D) and newly synthesized hydroxyproline (E). (F) Representative western blots of type I collagen and cytoskeleton proteins in liver lysates from the therapeutic CDA-HFD NASH model. GAPDH is a loading control. (G and H) Representative images (G) and quantification (H) of ACTA2 immunofluorescence in livers; scale bars, 100 mm. Data in (D), (E), and (H) represent mean ± SEM. p value is calculated using one-way ANOVA. See also Figure S11.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

    Techniques: Blocking Assay, Gene Expression, Staining, Synthesized, Western Blot, Control

    Differential effects of pre- and post-loading urine samples on the expression of WISP1, MMP9, Runx2, and TGFβ in EO771 cells. Of note, pre-S & post-S = pre- & post-step aerobics, and pre-L & post-L = pre- and post-loading. The bar chart is based on 10 pairs of human samples and 10 pairs of mouse samples. (A&B) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to human post-step aerobics urine samples. (C&D) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to murine post-knee loading urine samples.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Mechanical stimulations can inhibit local and remote tumor progression by downregulating WISP1

    doi: 10.1096/fj.202000713RR

    Figure Lengend Snippet: Differential effects of pre- and post-loading urine samples on the expression of WISP1, MMP9, Runx2, and TGFβ in EO771 cells. Of note, pre-S & post-S = pre- & post-step aerobics, and pre-L & post-L = pre- and post-loading. The bar chart is based on 10 pairs of human samples and 10 pairs of mouse samples. (A&B) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to human post-step aerobics urine samples. (C&D) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to murine post-knee loading urine samples.

    Article Snippet: We used antibodies against Runx2 (8486s), Snail (3879s), TGFβ (3711s) (Cell Signaling, Danvers, MA, USA), WISP1 (AF1689, R&D_Systems, Minneapolis, MN, USA), MMP9 (sc-393859) (Santa Cruz, Dallas, TX, USA), and β-actin (A5441, Sigma, Saint Louis, MO, USA).

    Techniques: Expressing

    Load-driven downregulation of WISP1 in the post-loading murine urine. Of note, CN = control, and VD = vitamin D3 (calcitriol). (A) Expression of 111 cytokines and chemokines in the mouse XL cytokine array. (B-D) WISP1-driven stimulation of EdU-based proliferation, Transwell invasion, and cellular motility of EO771 cells. (E) Elevation of MMP9, Runx2, TGFβ, and Snail by WISP1 in EO771 cells. (F) Size changes in fragmented breast cancer tumor tissue by calcitriol and WISP1.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Mechanical stimulations can inhibit local and remote tumor progression by downregulating WISP1

    doi: 10.1096/fj.202000713RR

    Figure Lengend Snippet: Load-driven downregulation of WISP1 in the post-loading murine urine. Of note, CN = control, and VD = vitamin D3 (calcitriol). (A) Expression of 111 cytokines and chemokines in the mouse XL cytokine array. (B-D) WISP1-driven stimulation of EdU-based proliferation, Transwell invasion, and cellular motility of EO771 cells. (E) Elevation of MMP9, Runx2, TGFβ, and Snail by WISP1 in EO771 cells. (F) Size changes in fragmented breast cancer tumor tissue by calcitriol and WISP1.

    Article Snippet: We used antibodies against Runx2 (8486s), Snail (3879s), TGFβ (3711s) (Cell Signaling, Danvers, MA, USA), WISP1 (AF1689, R&D_Systems, Minneapolis, MN, USA), MMP9 (sc-393859) (Santa Cruz, Dallas, TX, USA), and β-actin (A5441, Sigma, Saint Louis, MO, USA).

    Techniques: Control, Expressing

    WISP1 expression in TCGA and GTEx databases. (A) Elevated level of WISP1 transcripts in patients with breast cancer. (B) Significant reduction in the overall survival rate for patients with a high level of WISP1. (C) Correlation of transcript levels among WISP1, TGFβ, Runx2, and Snail in skeletal tissues. (D) Correlation of transcript levels among WISP1, TGFβ, Runx2, and MMP9 in breast cancer tissues. (E) Proposed mechanism for load-driven suppression of tumor progression, in which knee loading upregulated vitamin D3 (calcitriol) and downregulated cholesterol and TGFβ, which downregulated WISP1, MMP9, and Snail.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Mechanical stimulations can inhibit local and remote tumor progression by downregulating WISP1

    doi: 10.1096/fj.202000713RR

    Figure Lengend Snippet: WISP1 expression in TCGA and GTEx databases. (A) Elevated level of WISP1 transcripts in patients with breast cancer. (B) Significant reduction in the overall survival rate for patients with a high level of WISP1. (C) Correlation of transcript levels among WISP1, TGFβ, Runx2, and Snail in skeletal tissues. (D) Correlation of transcript levels among WISP1, TGFβ, Runx2, and MMP9 in breast cancer tissues. (E) Proposed mechanism for load-driven suppression of tumor progression, in which knee loading upregulated vitamin D3 (calcitriol) and downregulated cholesterol and TGFβ, which downregulated WISP1, MMP9, and Snail.

    Article Snippet: We used antibodies against Runx2 (8486s), Snail (3879s), TGFβ (3711s) (Cell Signaling, Danvers, MA, USA), WISP1 (AF1689, R&D_Systems, Minneapolis, MN, USA), MMP9 (sc-393859) (Santa Cruz, Dallas, TX, USA), and β-actin (A5441, Sigma, Saint Louis, MO, USA).

    Techniques: Expressing